Biolistics /gene gun is an effective method for the introduction of
desired DNA into the target site. It uses shock wave as the source of
force for the penetration of the micro-carrier (DNA coated on gold
nanoparticles) into the target cell. The integration of the gene of
interest(GOI) takes place through illegitimate recombination (i.e.) the
integration is not based on sequence homology. The greatest advantage of
exploiting gene gun for the integration of GOI is that "clean DNA" i.e.
GOI without the vector backbone can be inserted into the target genome.
This phenomenon reduces the risk of gene silencing that is partly
contributed by the vector backbone. On top of it, there is no need to
handle Agrobacterium tumifaciens and Biolistics is a rapid method as GOI
can be perfectly integrated within a matter of 5-6 hours.
FOODCROPS. HỌC MỖI NGÀY
No comments:
Post a Comment